Culture in vitro of orchids
Cultivation Tips for Orchids
Orchid Technical Sheets: Watering, Fertilizing, Substrate, Light
My Plants
Cloning
Cloning will get the same, the plant used for this operation. This is useful if you want to keep a particular strain but this operation is delicate.
my recommendations
Do not attempt cloning if:
You never do in vitro culture
You are new in vitro culture
You do not bloom
The shaft chosen for this operation is interrupted for more than 48 hours
The shaft is weak signs of illness, wrinkled, etc ...
The plant is infected by a virus
You can try cloning if:
you are comfortable with in vitro culture
you master the blooms of your plants and refloraisons
the staff chooses has all the necessary characteristics for a good cloning
1 - Prepare your environment by KOFF MEDIA4 ( with agar-agar) + coal in 1 liter of water.
2 - Sterilize your preparation with the cards present on the site
3 - Remove the stem open flowers if any remain. We must choose a clean flower stalk, healthy and vigorous, with terminal buds formed. The stems that work best are those which only the first flowers open. Avoid old withered stalks.
4 - Rinse the stem under running water for 5 minutes.
5 - Prepare a solution of bleach diluted to 10% and add 2 to 3 drops of additive.
6 - Cut the stem portion each including a node, using a razor blade or scalpel. Each portion should be 12-20 mm, retaining approximately 6mm above and below the node.
7 - Soak for 15 minutes the pieces so prepared in the bleach solution made in step 3, shaking regularly every 2-3 minutes.
8 - After this time, remove the stem portions of bleach (which can throw) and carefully remove the bracts that protect the dormant buds, aseptically.
9 - Prepare fresh solution of bleach diluted to 5% this time, and add 2-3 drops of additive.
10 - Once stripped of their bract, dip the stems in the bleach solution of 5%. Swim 10 minutes stirring regularly, every 2-3 minutes.
11 - This period of time has elapsed, remove the bleach rods and follow them in sterile water, stir well then empty. Repeat this step 3 times (3 different baths with renewed water).
12 - Remove yet 3mm rod before and after the bud, using a scalpel
13 - is then transferred sections thus obtained into containers in culture, prepared with Orchid Multiplication Medium. Other media, however, can be tempted (P748, B141, B142). Planting the longest portion of the stem in the middle, leaning slightly, so that the bud flush with the surface. In this way, the future grows normally point to the top emerging.
14 - In most cases, new growth should appear in a month and are usually ready to be transplanted had after 60 days.
15 - ften, nodes reject the phenolic acid in the medium becomes darker at the base of the stem. This acid is toxic to young growth that will then transplanted into a new environment of the fault perish. The use of an activated carbon containing medium reduced the frequency of subcultures because the carbon adsorbs and destroys phenolic compounds.
16 - Subculture in a maintenance medium (Orchid Maintenance Medium or P748 BM-1 / B141 or BM 2 / B142) and let the seedling grow and develop its root system. The roots usually appear after developing the first 2-3 leaves.
All the steps from 5 to 16 will be sterile glove-box. To sterilize it, thank you to consult the previous cards